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ARNTL Kit ELISA

ARNTL Reactivité: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
N° du produit ABIN5652030
  • Antigène Voir toutes ARNTL Kits ELISA
    ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))
    Reactivité
    • 4
    • 2
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL). No significant cross-reactivity or interference between Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL) and analogues was observed.
    Sensibilité
    0.057 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes ARNTL Kits ELISA
    ARNTL (Aryl Hydrocarbon Receptor Nuclear Translocator-Like (ARNTL))
    Autre désignation
    Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein (ARNTL Produits)
    Synonymes
    BMAL1 Kit ELISA, BMAL1c Kit ELISA, JAP3 Kit ELISA, MOP3 Kit ELISA, PASD3 Kit ELISA, TIC Kit ELISA, bHLHe5 Kit ELISA, Bmal1 Kit ELISA, ARNTL Kit ELISA, xBmal1 Kit ELISA, arntl Kit ELISA, Arnt3 Kit ELISA, BMAL1b Kit ELISA, bmal1b' Kit ELISA, aryl hydrocarbon receptor nuclear translocator like Kit ELISA, aryl hydrocarbon receptor nuclear translocator-like Kit ELISA, aryl hydrocarbon receptor nuclear translocator like S homeolog Kit ELISA, ARNTL Kit ELISA, Arntl Kit ELISA, arntl.S Kit ELISA, arntl Kit ELISA
    Sujet

    Gene Name: Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein

    Gene Aliases: BMAL1, BMAL1c, JAP3, MOP3, PASD3, TIC, BHLHE5, Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5

    ID gène
    29657
    UniProt
    Q9EPW1
    Pathways
    Regulation of Lipid Metabolism by PPARalpha, Protein targeting to Nucleus, L'effet Warburg
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