AHRR Kit ELISA

N° du produit ABIN5652031

Détail du produit AHRR Kit ELISA

Antigène: AHRR (Aryl-Hydrocarbon Receptor Repressor (AHRR))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Aryl Hydrocarbon Receptor Repressor (AHRR). No significant cross-reactivity or interference between Aryl Hydrocarbon Receptor Repressor (AHRR) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor Repressor (AHRR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor Repressor (AHRR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aryl Hydrocarbon Receptor Repressor (AHRR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aryl Hydrocarbon Receptor Repressor (AHRR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Repressor (AHRR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Repressor (AHRR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: AHRR (Aryl-Hydrocarbon Receptor Repressor (AHRR))

Autre désignation: Aryl Hydrocarbon Receptor Repressor (AHRR Produits)

Synonymes: mKIAA1234 Kit ELISA, AHH Kit ELISA, AHHR Kit ELISA, bHLHe77 Kit ELISA, ahh Kit ELISA, ahhr Kit ELISA, bhlhe77 Kit ELISA, ahrr2 Kit ELISA, aryl-hydrocarbon receptor repressor Kit ELISA, aryl hydrocarbon receptor repressor-like Kit ELISA, uncharacterized LOC723045 Kit ELISA, aryl-hydrocarbon receptor repressor L homeolog Kit ELISA, aryl hydrocarbon receptor repressor Kit ELISA, aryl-hydrocarbon receptor repressor b Kit ELISA, Ahrr Kit ELISA, AHRR Kit ELISA, LOC529500 Kit ELISA, LOC723045 Kit ELISA, ahrr.L Kit ELISA, LOC100455869 Kit ELISA, ahrr Kit ELISA, LOC100136487 Kit ELISA, ahrrb Kit ELISA

Sujet:

Gene Name: Aryl Hydrocarbon Receptor Repressor

Gene Aliases: AHH, AHHR, BHLHE77, Aryl Hydrocarbon Receptor Regulator, Class E basic helix-loop-helix protein 77

ID gène: 57491

UniProt: A9YTQ3

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha