Aspartate beta Hydroxylase Kit ELISA

N° du produit ABIN5652048

Détail du produit Aspartate beta Hydroxylase Kit ELISA

Antigène: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Aspartate Beta Hydroxylase (ASPH). No significant cross-reactivity or interference between Aspartate Beta Hydroxylase (ASPH) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Beta Hydroxylase (ASPH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aspartate Beta Hydroxylase (ASPH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Beta Hydroxylase (ASPH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aspartate Beta Hydroxylase (ASPH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Beta Hydroxylase (ASPH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Beta Hydroxylase (ASPH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Aspartate beta Hydroxylase (ASPH) (Aspartate beta-Hydroxylase (ASPH))

Autre désignation: Aspartate Beta Hydroxylase (ASPH Produits)

Synonymes: AAH Kit ELISA, BAH Kit ELISA, CASQ2BP1 Kit ELISA, HAAH Kit ELISA, JCTN Kit ELISA, junctin Kit ELISA, 2310005F16Rik Kit ELISA, 3110001L23Rik Kit ELISA, AI848629 Kit ELISA, AW261690 Kit ELISA, AW561901 Kit ELISA, C79816 Kit ELISA, cI-37 Kit ELISA, aah Kit ELISA, bah Kit ELISA, jctn Kit ELISA, MGC107896 Kit ELISA, cb971 Kit ELISA, fb69e10 Kit ELISA, fc06d04 Kit ELISA, fc95a08 Kit ELISA, wu:fb69e10 Kit ELISA, wu:fc06d04 Kit ELISA, wu:fc95a08 Kit ELISA, aspartate beta-hydroxylase Kit ELISA, aspartate-beta-hydroxylase Kit ELISA, aspartate beta-hydroxylase L homeolog Kit ELISA, ASPH Kit ELISA, Asph Kit ELISA, asph Kit ELISA, asph.L Kit ELISA

Sujet:

Gene Name: Aspartate Beta Hydroxylase

Gene Aliases: BAH, CASQ2BP1, HAAH, JCTN, Junctin, Humbug, Junctate, Aspartyl/Asparaginyl Beta-Hydroxylase, Peptide-aspartate beta-dioxygenase

ID gène: 444

UniProt: Q12797

Pathways: Positive Regulation of Endopeptidase Activity