ATG12 Kit ELISA

N° du produit ABIN5652118

Détail du produit ATG12 Kit ELISA

Antigène: ATG12 (Autophagy Related 12 (ATG12))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Autophagy Related Protein 12 (ATG12). No significant cross-reactivity or interference between Autophagy Related Protein 12 (ATG12) and analogues was observed.

Sensibilité: 13.1 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 12 (ATG12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 12 (ATG12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 12 (ATG12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 12 (ATG12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autophagy Related Protein 12 (ATG12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autophagy Related Protein 12 (ATG12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ATG12 (Autophagy Related 12 (ATG12))

Autre désignation: Autophagy Related Protein 12 (ATG12 Produits)

Synonymes: APG12 Kit ELISA, APG12L Kit ELISA, FBR93 Kit ELISA, HAPG12 Kit ELISA, Apg12l Kit ELISA, AUTOPHAGY 12 Kit ELISA, AUTOPHAGY 12 B Kit ELISA, CG10861 Kit ELISA, Dmel\\CG10861 Kit ELISA, atg12 Kit ELISA, ATG12 Kit ELISA, apg12 Kit ELISA, DDBDRAFT_0185323 Kit ELISA, DDBDRAFT_0191412 Kit ELISA, DDB_0185323 Kit ELISA, DDB_0191412 Kit ELISA, 4931423H11Rik Kit ELISA, A330058M13Rik Kit ELISA, Atg12l Kit ELISA, AO090001000593 Kit ELISA, autophagy related 12 Kit ELISA, Ubiquitin-like superfamily protein Kit ELISA, Atg12p Kit ELISA, autophagy protein Kit ELISA, ubiquitin-like protein ATG12B Kit ELISA, autophagy associated protein Atg12 Kit ELISA, Autophagy-related 12 Kit ELISA, ATG12 autophagy related 12 homolog (S. cerevisiae) Kit ELISA, ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene Kit ELISA, autophagy associated ubiquitin-like modifier Atg12 Kit ELISA, hypothetical protein Kit ELISA, Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12) Kit ELISA, autophagy protein 12 Kit ELISA, autophagy related 12 S homeolog Kit ELISA, autophagy-related protein 12 Kit ELISA, ATG12 Kit ELISA, Atg12 Kit ELISA, APG12B Kit ELISA, APG12 Kit ELISA, LOC9321066 Kit ELISA, apg12 Kit ELISA, atg12 Kit ELISA, LOC743425 Kit ELISA, CAALFM_C113950CA Kit ELISA, atg12.S Kit ELISA, ANI_1_922094 Kit ELISA, AOR_1_1064164 Kit ELISA

Sujet:

Gene Name: Autophagy Related Protein 12

Gene Aliases: APG12, APG12L, HAPG12, Ubiquitin-like protein ATG12

Pathways: Autophagy