ATG16L1 Kit ELISA

N° du produit ABIN5652119

Détail du produit ATG16L1 Kit ELISA

Antigène: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Autophagy Related Protein 16 Like Protein 1 (ATG16L1). No significant cross-reactivity or interference between Autophagy Related Protein 16 Like Protein 1 (ATG16L1) and analogues was observed.

Sensibilité: 0.063 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 16 Like Protein 1 (ATG16L1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 16 Like Protein 1 (ATG16L1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 16 Like Protein 1 (ATG16L1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 16 Like Protein 1 (ATG16L1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autophagy Related Protein 16 Like Protein 1 (ATG16L1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autophagy Related Protein 16 Like Protein 1 (ATG16L1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ATG16L1 (ATG16 Autophagy Related 16-Like 1 (ATG16L1))

Autre désignation: Autophagy Related Protein 16 Like Protein 1 (ATG16L1 Produits)

Synonymes: APG16L Kit ELISA, ATG16A Kit ELISA, ATG16L Kit ELISA, IBD10 Kit ELISA, WDR30 Kit ELISA, 1500009K01Rik Kit ELISA, Apg16l Kit ELISA, Atg16l Kit ELISA, Wdr30 Kit ELISA, atg16 Kit ELISA, atg16l Kit ELISA, fb57e05 Kit ELISA, wu:fb57e05 Kit ELISA, zgc:110147 Kit ELISA, autophagy related 16 like 1 Kit ELISA, autophagy related 16-like 1 (S. cerevisiae) Kit ELISA, autophagy related 16-like 1 Kit ELISA, autophagy related 16 like 1 S homeolog Kit ELISA, ATG16 autophagy related 16-like 1 (S. cerevisiae) Kit ELISA, ATG16L1 Kit ELISA, Atg16l1 Kit ELISA, atg16l1 Kit ELISA, atg16l1.S Kit ELISA

Sujet:

Gene Name: Autophagy Related Protein 16 Like Protein 1

Gene Aliases: WDR30

ID gène: 55054

UniProt: Q676U5

Pathways: Autophagy