ATG5 Kit ELISA

N° du produit ABIN5652120

Détail du produit ATG5 Kit ELISA

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Autophagy Related Protein 5 (ATG5). No significant cross-reactivity or interference between Autophagy Related Protein 5 (ATG5) and analogues was observed.

Sensibilité: 0.28 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 5 (ATG5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 5 (ATG5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 5 (ATG5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 5 (ATG5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autophagy Related Protein 5 (ATG5) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autophagy Related Protein 5 (ATG5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Autre désignation: Autophagy Related Protein 5 (ATG5 Produits)

Synonymes: APG5 Kit ELISA, APG5-LIKE Kit ELISA, APG5L Kit ELISA, ASP Kit ELISA, hAPG5 Kit ELISA, ATG5 Kit ELISA, CG1643 Kit ELISA, DmAtg5 Kit ELISA, Dmel\\CG1643 Kit ELISA, atg5 Kit ELISA, 2010107M05Rik Kit ELISA, 3110067M24Rik Kit ELISA, AW319544 Kit ELISA, Apg5l Kit ELISA, Atg5l Kit ELISA, C88337 Kit ELISA, Paddy Kit ELISA, apg5l Kit ELISA, zgc:100934 Kit ELISA, ATATG5 Kit ELISA, AUTOPHAGY 5 Kit ELISA, MKP11.20 Kit ELISA, MKP11_20 Kit ELISA, autophagy related 5 Kit ELISA, Autophagy-related 5 Kit ELISA, ATG5 autophagy related 5 homolog (S. cerevisiae) Kit ELISA, autophagy related 5 L homeolog Kit ELISA, similar to S. cerevisiae ATG5 (YPL149W) which nucleates preautophagosome formation as a conjugate with Atg12 Kit ELISA, autophagy protein Apg5 family Kit ELISA, ATG5 Kit ELISA, Atg5 Kit ELISA, atg5 Kit ELISA, atg5.L Kit ELISA, APG5 Kit ELISA

Sujet:

Gene Name: Autophagy Related Protein 5

Gene Aliases: ASP, APG5, APG5L, hAPG5, Apoptosis-specific protein, Autophagy protein 5

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy