ATG7 Kit ELISA

N° du produit ABIN5652122

Détail du produit ATG7 Kit ELISA

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Autophagy Related Protein 7 (ATG7). No significant cross-reactivity or interference between Autophagy Related Protein 7 (ATG7) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 7 (ATG7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Autophagy Related Protein 7 (ATG7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 7 (ATG7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 7 (ATG7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Autophagy Related Protein 7 (ATG7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Autophagy Related Protein 7 (ATG7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ATG7 (ATG7 Autophagy Related 7 (ATG7))

Autre désignation: Autophagy Related Protein 7 (ATG7 Produits)

Synonymes: APG7-LIKE Kit ELISA, APG7L Kit ELISA, GSA7 Kit ELISA, D8 Kit ELISA, UBA1B Kit ELISA, UBE1L Kit ELISA, UBE2 Kit ELISA, Ube1l Kit ELISA, 1300004C08Rik Kit ELISA, AO090001000744 Kit ELISA, atg7 Kit ELISA, 1810013K23Rik Kit ELISA, Agp7 Kit ELISA, Apg7l Kit ELISA, Atg7l Kit ELISA, ATAPG7 Kit ELISA, ATATG7 Kit ELISA, ATG7 Kit ELISA, AUTOPHAGY 7 Kit ELISA, AUTOPHAGY-RELATED 7 Kit ELISA, K15I22.10 Kit ELISA, K15I22_10 Kit ELISA, autophagy related 7 Kit ELISA, ubiquitin like modifier activating enzyme 7 Kit ELISA, ubiquitin-like modifier activating enzyme 7 Kit ELISA, autophagy-related protein 7 Kit ELISA, Autophagy-related protein 7 Kit ELISA, Apg7p Kit ELISA, ThiF family protein Kit ELISA, ubiquitin-like conjugating enzyme Atg7 Kit ELISA, ATG7 Kit ELISA, UBA7 Kit ELISA, Uba7 Kit ELISA, ANI_1_3368024 Kit ELISA, AOR_1_1338164 Kit ELISA, PTRG_02236 Kit ELISA, PAAG_00287 Kit ELISA, PITG_12537 Kit ELISA, atg7 Kit ELISA, Atg7 Kit ELISA, APG7 Kit ELISA

Sujet:

Gene Name: Autophagy Related Protein 7

Gene Aliases: APG7L, GSA7, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Ubiquitin-activating enzyme E1-like protein

ID gène: 74244

UniProt: Q9D906

Pathways: Response to Water Deprivation, Autophagy