BAG3 Kit ELISA

N° du produit ABIN5652163

Détail du produit BAG3 Kit ELISA

Antigène: BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Bcl2 Associated Athanogene 3 (BAG3). No significant cross-reactivity or interference between Bcl2 Associated Athanogene 3 (BAG3) and analogues was observed.

Sensibilité: 0.061 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated Athanogene 3 (BAG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Associated Athanogene 3 (BAG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated Athanogene 3 (BAG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bcl2 Associated Athanogene 3 (BAG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Associated Athanogene 3 (BAG3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Associated Athanogene 3 (BAG3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BAG3 (BCL2-Associated Athanogene 3 (BAG3))

Autre désignation: Bcl2 Associated Athanogene 3 (BAG3 Produits)

Synonymes: bag3-A Kit ELISA, MGC53113 Kit ELISA, zgc:100859 Kit ELISA, BAG3 Kit ELISA, ATBAG3 Kit ELISA, BCL-2-associated athanogene 3 Kit ELISA, T28J14.160 Kit ELISA, T28J14_160 Kit ELISA, BAG-3 Kit ELISA, BIS Kit ELISA, CAIR-1 Kit ELISA, MFM6 Kit ELISA, AA407278 Kit ELISA, Bis Kit ELISA, mg638 Kit ELISA, BCL2 associated athanogene 3 L homeolog Kit ELISA, BCL2-associated athanogene 3 Kit ELISA, BCL2 associated athanogene 3 Kit ELISA, BCL-2-associated athanogene 3 Kit ELISA, Bcl2-associated athanogene 3 Kit ELISA, bag3.L Kit ELISA, bag3 Kit ELISA, BAG3 Kit ELISA, Bag3 Kit ELISA

Sujet:

Gene Name: Bcl2 Associated Athanogene 3

Gene Aliases: BIS, CAIR1, BAG Family Molecular Chaperone Regulator 3, Bcl-2-binding protein Bis, Docking protein CAIR-1