Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Big Dynorphin Kit ELISA

Reactivité: Humain Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Plasma, Serum
N° du produit ABIN5652222
  • Antigène Tous les produits Big Dynorphin
    Big Dynorphin
    Reactivité
    • 3
    • 3
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    6.17 pg/mL - 500 pg/mL
    Seuil minimal de détection
    6.17 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Big Dynorphin (Dyn). No significant cross-reactivity or interference between Big Dynorphin (Dyn) and analogues was observed.
    Sensibilité
    2.56 pg/mL
    Featured
    Discover our best selling Big Dynorphin Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Big Dynorphin (Dyn) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Big Dynorphin (Dyn) and unlabeled Big Dynorphin (Dyn) (Standards or samples) with the pre-coated antibody specific to Big Dynorphin (Dyn). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Big Dynorphin (Dyn) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Big Dynorphin (Dyn) in the sample.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Big Dynorphin (Dyn) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Big Dynorphin (Dyn) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Tous les produits Big Dynorphin
    Big Dynorphin
    Abstract
    Big Dynorphin Produits
    Synonymes
    ADCA Kit ELISA, PENKB Kit ELISA, SCA23 Kit ELISA, Dyn Kit ELISA, prodynorphin Kit ELISA, PDYN Kit ELISA, Pdyn Kit ELISA
    Sujet

    Gene Name: Big Dynorphin

    Gene Aliases: B-DYN, Biocytin 13 Dynorphin A 1-13 Amide

Vous êtes ici:
Support technique