Biliverdin Reductase Kit ELISA

N° du produit ABIN5652229

Détail du produit Biliverdin Reductase Kit ELISA

Antigène: Biliverdin Reductase (BLVRA) (Biliverdin Reductase A (BLVRA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.625 ng/mL - 40 ng/mL

Seuil minimal de détection: 0.625 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Biliverdin Reductase A (BLVRA). No significant cross-reactivity or interference between Biliverdin Reductase A (BLVRA) and analogues was observed.

Sensibilité: 0.273 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Biliverdin Reductase A (BLVRA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Biliverdin Reductase A (BLVRA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Biliverdin Reductase A (BLVRA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Biliverdin Reductase A (BLVRA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Biliverdin Reductase A (BLVRA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Biliverdin Reductase A (BLVRA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Biliverdin Reductase (BLVRA) (Biliverdin Reductase A (BLVRA))

Autre désignation: Biliverdin Reductase A (BLVRA Produits)

Synonymes: blvra Kit ELISA, MGC69310 Kit ELISA, BLVRA Kit ELISA, zgc:153046 Kit ELISA, BVR Kit ELISA, Dmel\\CG9471 Kit ELISA, BLVR Kit ELISA, BVRA Kit ELISA, 0610006A11Rik Kit ELISA, 2500001N03Rik Kit ELISA, Blvr Kit ELISA, biliverdin reductase A Kit ELISA, biliverdin reductase A L homeolog Kit ELISA, CG9471 gene product from transcript CG9471-RA Kit ELISA, BLVRA Kit ELISA, blvra Kit ELISA, blvra.L Kit ELISA, CG9471 Kit ELISA, Blvra Kit ELISA

Sujet:

Gene Name: Biliverdin Reductase A

Gene Aliases: BLVR, BVR, Biliverdin-IX alpha-reductase

ID gène: 644

UniProt: P53004