BAMBI Kit ELISA

N° du produit ABIN5652235

Détail du produit BAMBI Kit ELISA

Antigène: BAMBI (BMP and Activin Membrane-Bound Inhibitor Homolog (BAMBI))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI). No significant cross-reactivity or interference between BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BAMBI (BMP and Activin Membrane-Bound Inhibitor Homolog (BAMBI))

Autre désignation: BMP And Activin Membrane Bound Inhibitor Homolog (BAMBI Produits)

Synonymes: NMA Kit ELISA, bambi Kit ELISA, nma Kit ELISA, BAMBI Kit ELISA, Zfnma Kit ELISA, cb998 Kit ELISA, fc44c12 Kit ELISA, id:ibd5020 Kit ELISA, wu:fc44c12 Kit ELISA, wu:fe01e03 Kit ELISA, 2610003H06Rik Kit ELISA, bambi-A Kit ELISA, zgc:91871 Kit ELISA, BMP and activin membrane bound inhibitor Kit ELISA, BMP and activin membrane-bound inhibitor homolog Kit ELISA, BMP and activin membrane-bound inhibitor Kit ELISA, BMP and activin membrane-bound inhibitor (Xenopus laevis) homolog a Kit ELISA, BMP and activin membrane-bound inhibitor L homeolog Kit ELISA, BMP and activin membrane-bound inhibitor homolog (Xenopus laevis) b Kit ELISA, BAMBI Kit ELISA, bambi Kit ELISA, bambia Kit ELISA, Bambi Kit ELISA, LOC724378 Kit ELISA, LOC100647353 Kit ELISA, bambi.L Kit ELISA, bambib Kit ELISA

Sujet:

Gene Name: BMP And Activin Membrane Bound Inhibitor Homolog

Gene Aliases: NMA, Non-metastatic gene A protein, Putative transmembrane protein NMA

ID gène: 25805

UniProt: Q13145