BMPR1A Kit ELISA

N° du produit ABIN5652298

Détail du produit BMPR1A Kit ELISA

Antigène: BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein Receptor 1A (BMPR1A). No significant cross-reactivity or interference between Bone Morphogenetic Protein Receptor 1A (BMPR1A) and analogues was observed.

Sensibilité: 0.24 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein Receptor 1A (BMPR1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein Receptor 1A (BMPR1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bone Morphogenetic Protein Receptor 1A (BMPR1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bone Morphogenetic Protein Receptor 1A (BMPR1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1A (BMPR1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bone Morphogenetic Protein Receptor 1A (BMPR1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))

Autre désignation: Bone Morphogenetic Protein Receptor 1A (BMPR1A Produits)

Synonymes: 10q23del Kit ELISA, ACVRLK3 Kit ELISA, ALK3 Kit ELISA, CD292 Kit ELISA, SKR5 Kit ELISA, 1110037I22Rik Kit ELISA, AU045487 Kit ELISA, BMPR-IA Kit ELISA, Bmpr Kit ELISA, BMPR-1 Kit ELISA, BRK-1 Kit ELISA, BMPR1A Kit ELISA, acvrlk3 Kit ELISA, alk-3 Kit ELISA, alk3 Kit ELISA, cd292 Kit ELISA, skr5 Kit ELISA, BR1a Kit ELISA, alk3tr Kit ELISA, bmpr1a Kit ELISA, zgc:136731 Kit ELISA, wu:fe05b06 Kit ELISA, wu:fe06e10 Kit ELISA, zgc:92227 Kit ELISA, bone morphogenetic protein receptor type 1A Kit ELISA, bone morphogenetic protein receptor, type 1A Kit ELISA, bone morphogenetic protein receptor type 1A S homeolog Kit ELISA, bone morphogenetic protein receptor, type IAa Kit ELISA, bone morphogenetic protein receptor, type IAb Kit ELISA, BMPR1A Kit ELISA, Bmpr1a Kit ELISA, bmpr1a Kit ELISA, bmpr1a.S Kit ELISA, bmpr1aa Kit ELISA, bmpr1ab Kit ELISA

Sujet:

Gene Name: Bone Morphogenetic Protein Receptor 1A

Gene Aliases: CD292, ACVRLK3, ALK3, SKR5, BMPR1-A, Activin A Receptor, Type Ii-Like Kinase 3, Activin Receptor-Like Kinase 3, Serine/Threonine-Protein Kinase Receptor R5

ID gène: 81507

UniProt: Q78EA7

Pathways: Stem Cell Maintenance