BCKDK Kit ELISA

N° du produit ABIN5652334

Détail du produit BCKDK Kit ELISA

Antigène: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Seuil minimal de détection: 3.12 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK). No significant cross-reactivity or interference between Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) and analogues was observed.

Sensibilité: 1.29 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BCKDK (Branched Chain Ketoacid Dehydrogenase Kinase (BCKDK))

Autre désignation: Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK Produits)

Synonymes: cb233 Kit ELISA, zgc:55984 Kit ELISA, wu:fa04g07 Kit ELISA, wu:fb80b08 Kit ELISA, MGC78818 Kit ELISA, BCKDK Kit ELISA, AI327402 Kit ELISA, BCKDKD Kit ELISA, BDK Kit ELISA, branched chain ketoacid dehydrogenase kinase Kit ELISA, branched chain ketoacid dehydrogenase kinase S homeolog Kit ELISA, bckdk Kit ELISA, bckdk.S Kit ELISA, BCKDK Kit ELISA, Bckdk Kit ELISA

Sujet:

Gene Name: Branched Chain Alpha-Ketoacid Dehydrogenase Kinase

Gene Aliases: BCKDHKIN, 3-Methyl-2-Oxobutanoate Dehydrogenase/Lipoamide Kinase,Mitochondrial

ID gène: 29603

UniProt: Q00972

Pathways: SARS-CoV-2 Protein Interactome