BRD4 Kit ELISA

N° du produit ABIN5652353

Détail du produit BRD4 Kit ELISA

Antigène: BRD4 (Bromodomain Containing 4 (BRD4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Bromodomain Containing Protein 4 (BRD4). No significant cross-reactivity or interference between Bromodomain Containing Protein 4 (BRD4) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bromodomain Containing Protein 4 (BRD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bromodomain Containing Protein 4 (BRD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bromodomain Containing Protein 4 (BRD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bromodomain Containing Protein 4 (BRD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bromodomain Containing Protein 4 (BRD4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bromodomain Containing Protein 4 (BRD4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: BRD4 (Bromodomain Containing 4 (BRD4))

Autre désignation: Bromodomain Containing Protein 4 (BRD4 Produits)

Synonymes: brd4-b Kit ELISA, BRD4 Kit ELISA, fb18g03 Kit ELISA, wu:fb18g03 Kit ELISA, CAP Kit ELISA, HUNK1 Kit ELISA, HUNKI Kit ELISA, MCAP Kit ELISA, Brd5 Kit ELISA, WI-11513 Kit ELISA, bromodomain containing 4 L homeolog Kit ELISA, bromodomain containing 4 Kit ELISA, bromodomain-containing protein 4 Kit ELISA, brd4.L Kit ELISA, BRD4 Kit ELISA, brd4 Kit ELISA, LOC100409351 Kit ELISA, Brd4 Kit ELISA

Sujet:

Gene Name: Bromodomain Containing Protein 4

Gene Aliases: CAP, HUNKI, MCAP, HUNK1, Chromosome-Associated Protein

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome