Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

OB Cadherin Kit ELISA

CDH11 Reactivité: Humain Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
N° du produit ABIN5652463
  • Antigène Voir toutes OB Cadherin (CDH11) Kits ELISA
    OB Cadherin (CDH11) (Cadherin 11 (CDH11))
    Reactivité
    • 4
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.312 ng/mL - 20 ng/mL
    Seuil minimal de détection
    0.312 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Cadherin, Osteoblast (CDHOB). No significant cross-reactivity or interference between Cadherin, Osteoblast (CDHOB) and analogues was observed.
    Sensibilité
    0.116 ng/mL
    Featured
    Discover our best selling CDH11 Kit ELISA
    Top Product
    Discover our top product CDH11 Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cadherin, Osteoblast (CDHOB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cadherin, Osteoblast (CDHOB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cadherin, Osteoblast (CDHOB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cadherin, Osteoblast (CDHOB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cadherin, Osteoblast (CDHOB) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cadherin, Osteoblast (CDHOB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes OB Cadherin (CDH11) Kits ELISA
    OB Cadherin (CDH11) (Cadherin 11 (CDH11))
    Autre désignation
    Cadherin, Osteoblast (CDH11 Produits)
    Synonymes
    cad11 Kit ELISA, cdhob Kit ELISA, osf-4 Kit ELISA, Xcad-11 Kit ELISA, MGC108014 Kit ELISA, OB-cadherin Kit ELISA, cadherin-11 Kit ELISA, Cad11 Kit ELISA, cdhvn Kit ELISA, vnc Kit ELISA, CAD11 Kit ELISA, CDHOB Kit ELISA, OB Kit ELISA, OSF-4 Kit ELISA, cadherin 11 Kit ELISA, cadherin 11, type 2, OB-cadherin (osteoblast) Kit ELISA, cadherin 11 S homeolog Kit ELISA, CDH11 Kit ELISA, cdh11 Kit ELISA, Cdh11 Kit ELISA, cdh11.S Kit ELISA
    Sujet

    Gene Name: Cadherin, Osteoblast

    Gene Aliases: CDH11, CAD11, OB, OSF4, Cadherin 11 Type 2, OB-Cadherin

    ID gène
    1009
    UniProt
    P55287
Vous êtes ici:
Support technique