CASR Kit ELISA
-
- Antigène Voir toutes CASR Kits ELISA
- CASR (Calcium-Sensing Receptor (CASR))
-
Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Calcium Sensing Receptor (CASR). No significant cross-reactivity or interference between Calcium Sensing Receptor (CASR) and analogues was observed.
- Sensibilité
- 0.057 ng/mL
- Featured
- Discover our best selling CASR Kit ELISA
- Top Product
- Discover our top product CASR Kit ELISA
-
-
- Commentaires
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Sensing Receptor (CASR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Sensing Receptor (CASR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Sensing Receptor (CASR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Sensing Receptor (CASR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Sensing Receptor (CASR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Sensing Receptor (CASR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
-
- Antigène Voir toutes CASR Kits ELISA
- CASR (Calcium-Sensing Receptor (CASR))
- Autre désignation
- Calcium Sensing Receptor (CASR Produits)
- Synonymes
- CAR Kit ELISA, EIG8 Kit ELISA, FHH Kit ELISA, FIH Kit ELISA, GPRC2A Kit ELISA, HHC Kit ELISA, HHC1 Kit ELISA, HYPOC1 Kit ELISA, NSHPT Kit ELISA, PCAR1 Kit ELISA, CaR Kit ELISA, Gprc2a Kit ELISA, gprc2a Kit ELISA, xV2R F-1 Kit ELISA, CASR Kit ELISA, calcium sensing receptor Kit ELISA, calcium-sensing receptor Kit ELISA, calcium-sensing receptor L homeolog Kit ELISA, CASR Kit ELISA, Casr Kit ELISA, casr.L Kit ELISA
- Sujet
-
Gene Name: Calcium Sensing Receptor
Gene Aliases: CAR, FHH, FIH, GPRC2A, HHC, HHC1, NSHPT, PCAR1, Parathyroid cell calcium-sensing receptor 1, Hypocalciuric Hypercalcemia 1, Severe Neonatal Hyperparathyroidism
- ID gène
- 12374
- UniProt
- Q9QY96
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
-