Calpain 3 Kit ELISA

N° du produit ABIN5652515

Détail du produit Calpain 3 Kit ELISA

Antigène: Calpain 3 (CAPN3)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Calpain 3 (CAPN3). No significant cross-reactivity or interference between Calpain 3 (CAPN3) and analogues was observed.

Sensibilité: 0.125 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain 3 (CAPN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain 3 (CAPN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain 3 (CAPN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calpain 3 (CAPN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpain 3 (CAPN3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpain 3 (CAPN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Calpain 3 (CAPN3)

Autre désignation: Calpain 3 (CAPN3 Produits)

Synonymes: capn3 Kit ELISA, MGC80925 Kit ELISA, zgc:92687 Kit ELISA, CAPN3 Kit ELISA, AI323605 Kit ELISA, Capa-3 Kit ELISA, Capa3 Kit ELISA, Lp82 Kit ELISA, p94 Kit ELISA, Lp84 Kit ELISA, Lp85 Kit ELISA, CANP3 Kit ELISA, CANPL3 Kit ELISA, LGMD2 Kit ELISA, LGMD2A Kit ELISA, nCL-1 Kit ELISA, SKM-CALP Kit ELISA, SKM-CALPOV Kit ELISA, nCL1 Kit ELISA, calpain 1, (mu/I) large subunit L homeolog Kit ELISA, calpain 3a, (p94) Kit ELISA, calpain 3 Kit ELISA, capn1.L Kit ELISA, capn3a Kit ELISA, CAPN3 Kit ELISA, Capn3 Kit ELISA

Sujet:

Gene Name: Calpain 3

Gene Aliases: CANPL3, p94, LGMD2, LGMD2A, nCL1, Calcium-activated neutral proteinase 3, Muscle-specific calcium-activated neutral protease 3, New calpain 1

ID gène: 825

UniProt: P20807

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development