Calpain S1 Kit ELISA

N° du produit ABIN5652517

Détail du produit Calpain S1 Kit ELISA

Antigène: Calpain S1 (CAPNS1) (Calpain, Small Subunit 1 (CAPNS1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Calpain, Small Subunit 1 (CAPNS1). No significant cross-reactivity or interference between Calpain, Small Subunit 1 (CAPNS1) and analogues was observed.

Sensibilité: 0.126 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calpain, Small Subunit 1 (CAPNS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calpain, Small Subunit 1 (CAPNS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calpain, Small Subunit 1 (CAPNS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calpain, Small Subunit 1 (CAPNS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calpain, Small Subunit 1 (CAPNS1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calpain, Small Subunit 1 (CAPNS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Calpain S1 (CAPNS1) (Calpain, Small Subunit 1 (CAPNS1))

Autre désignation: Calpain, Small Subunit 1 (CAPNS1 Produits)

Synonymes: 30K Kit ELISA, CALPAIN4 Kit ELISA, CANP Kit ELISA, CANPS Kit ELISA, CAPN4 Kit ELISA, CDPS Kit ELISA, CSS1 Kit ELISA, Capn4 Kit ELISA, CAPNS1 Kit ELISA, Capa-4 Kit ELISA, Capa4 Kit ELISA, D7Ertd146e Kit ELISA, MGC84330 Kit ELISA, capns1 Kit ELISA, cb616 Kit ELISA, wu:fb81g08 Kit ELISA, wu:fl09e04 Kit ELISA, zgc:110603 Kit ELISA, calpain small subunit 1 Kit ELISA, calpain, small subunit 1 Kit ELISA, calpain, small subunit 1 L homeolog Kit ELISA, calpain, small subunit 1 a Kit ELISA, CAPNS1 Kit ELISA, Capns1 Kit ELISA, capns1.L Kit ELISA, capns1a Kit ELISA

Sujet:

Gene Name: Calpain, Small Subunit 1

Gene Aliases: CANP, 30K, Calpain 4, CANPS, CAPN4, CDPS, Calcium-activated neutral proteinase small subunit, Calcium-dependent protease small subunit, Calpain regulatory subunit

ID gène: 826

UniProt: P04632

Pathways: Apoptose