Calreticulin Kit ELISA
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- Antigène Voir toutes Calreticulin (CALR) Kits ELISA
- Calreticulin (CALR)
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156 ng/mL - 10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Calreticulin (CRT). No significant cross-reactivity or interference between Calreticulin (CRT) and analogues was observed.
- Sensibilité
- 0.067 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calreticulin (CRT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calreticulin (CRT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calreticulin (CRT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calreticulin (CRT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calreticulin (CRT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calreticulin (CRT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes Calreticulin (CALR) Kits ELISA
- Calreticulin (CALR)
- Autre désignation
- Calreticulin (CALR Produits)
- Synonymes
- CRT Kit ELISA, RO Kit ELISA, SSA Kit ELISA, cC1qR Kit ELISA, crc Kit ELISA, crt Kit ELISA, Calregulin Kit ELISA, CG9429 Kit ELISA, Clr Kit ELISA, Dmel\\CG9429 Kit ELISA, anon-EST:Liang-2.11 Kit ELISA, anon-EST:Liang-2.17 Kit ELISA, anon-EST:fe3D7 Kit ELISA, clone 2.11 Kit ELISA, clone 2.17 Kit ELISA, eth Kit ELISA, potp Kit ELISA, cc1qr Kit ELISA, ssa Kit ELISA, NV19007 Kit ELISA, CRP55 Kit ELISA, ERp60 Kit ELISA, HACBP Kit ELISA, CHOT1 Kit ELISA, CHT1 Kit ELISA, CT1 Kit ELISA, AA589632 Kit ELISA, CRTR Kit ELISA, Creat Kit ELISA, CCDS1 Kit ELISA, calrl2 Kit ELISA, fc66d10 Kit ELISA, wu:fc66d10 Kit ELISA, wu:fj50c03 Kit ELISA, zgc:66153 Kit ELISA, calreticulin Kit ELISA, calreticulin L homeolog Kit ELISA, Calreticulin Kit ELISA, putative calreticulin Kit ELISA, solute carrier family 6 member 8 Kit ELISA, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 Kit ELISA, CALR Kit ELISA, calr.L Kit ELISA, Crt Kit ELISA, crt Kit ELISA, CRT Kit ELISA, Calr Kit ELISA, calr Kit ELISA, LMJF_31_2600 Kit ELISA, Crc Kit ELISA, Slc6a8 Kit ELISA, SLC6A8 Kit ELISA, LOC8269812 Kit ELISA
- Sujet
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Gene Name: Calreticulin
Gene Aliases: CALR, RO, SSA, CC1qR, ERp60, HACBP, grp60, CRTC, CRP55, Calregulin, Sicca Syndrome Antigen A, Autoantigen Ro, Endoplasmic reticulum resident protein 60
- ID gène
- 811
- UniProt
- P27797
- Pathways
- Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response
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