Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CES2 Kit ELISA

CES2 Reactivité: Humain Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5652566
  • Antigène Voir toutes CES2 Kits ELISA
    CES2 (Carboxylesterase 2 (CES2))
    Reactivité
    • 5
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.625 ng/mL - 40 ng/mL
    Seuil minimal de détection
    0.625 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Carboxylesterase 2 (CES2). No significant cross-reactivity or interference between Carboxylesterase 2 (CES2) and analogues was observed.
    Sensibilité
    0.268 ng/mL
    Featured
    Discover our best selling CES2 Kit ELISA
    Top Product
    Discover our top product CES2 Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxylesterase 2 (CES2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxylesterase 2 (CES2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxylesterase 2 (CES2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxylesterase 2 (CES2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carboxylesterase 2 (CES2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carboxylesterase 2 (CES2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes CES2 Kits ELISA
    CES2 (Carboxylesterase 2 (CES2))
    Autre désignation
    Carboxylesterase 2 (CES2 Produits)
    Synonymes
    CE-2 Kit ELISA, CES2A1 Kit ELISA, PCE-2 Kit ELISA, iCE Kit ELISA, Ces2 Kit ELISA, im:6908784 Kit ELISA, si:dkey-38l12.2 Kit ELISA, zgc:153863 Kit ELISA, ce-2 Kit ELISA, ces2a1 Kit ELISA, pce-2 Kit ELISA, carboxylesterase 2 Kit ELISA, liver carboxylesterase 2 Kit ELISA, cocaine esterase Kit ELISA, carboxylesterase 2 (intestine, liver) Kit ELISA, CES2 Kit ELISA, Ces2 Kit ELISA, LOC100343300 Kit ELISA, LOC100734360 Kit ELISA, ces2 Kit ELISA
    Sujet

    Gene Name: Carboxylesterase 2

    Gene Aliases: CE-2, CES2A1, PCE-2, iCE, Intestine,Liver, Methylumbelliferyl-acetate deacetylase 2, Cocaine esterase

    ID gène
    8824
    UniProt
    O00748
Vous êtes ici:
Support technique