CRAT Kit ELISA

N° du produit ABIN5652607

Détail du produit CRAT Kit ELISA

Antigène: CRAT (Carnitine O-Acetyltransferase (CRAT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Seuil minimal de détection: 3.12 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Carnitine Acetyltransferase (CRAT). No significant cross-reactivity or interference between Carnitine Acetyltransferase (CRAT) and analogues was observed.

Sensibilité: 1.31 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Acetyltransferase (CRAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carnitine Acetyltransferase (CRAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carnitine Acetyltransferase (CRAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carnitine Acetyltransferase (CRAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carnitine Acetyltransferase (CRAT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carnitine Acetyltransferase (CRAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CRAT (Carnitine O-Acetyltransferase (CRAT))

Autre désignation: Carnitine Acetyltransferase (CRAT Produits)

Synonymes: CAT1 Kit ELISA, AW107812 Kit ELISA, CARAT Kit ELISA, CAT Kit ELISA, Carnitine acetylase Kit ELISA, CrAT Kit ELISA, hm:zeh0248 Kit ELISA, zgc:92317 Kit ELISA, carnitine O-acetyltransferase Kit ELISA, carnitine acetyltransferase Kit ELISA, Carnitine acetyltransferase Kit ELISA, Carnitine acetyltransferase, putative Kit ELISA, carnitine O-acetyltransferase a Kit ELISA, CRAT Kit ELISA, Crat Kit ELISA, CNA05200 Kit ELISA, CNL05760 Kit ELISA, CC1G_06292 Kit ELISA, PAS_chr3_0761 Kit ELISA, CGB_A5470W Kit ELISA, CGB_D1240C Kit ELISA, crata Kit ELISA

Sujet:

Gene Name: Carnitine Acetyltransferase

Gene Aliases: CAT1, Carnitine O-Acetyltransferase, Carnitine acetylase

ID gène: 1384

UniProt: P43155

Pathways: Monocarboxylic Acid Catabolic Process