Casein Kinase 1 delta Kit ELISA

N° du produit ABIN5652639

Détail du produit Casein Kinase 1 delta Kit ELISA

Antigène: Casein Kinase 1 delta (CSNK1D) (Casein Kinase 1, delta (CSNK1D))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Casein Kinase 1 Delta (CSNK1d). No significant cross-reactivity or interference between Casein Kinase 1 Delta (CSNK1d) and analogues was observed.

Sensibilité: 0.052 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Kinase 1 Delta (CSNK1d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Casein Kinase 1 Delta (CSNK1d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Kinase 1 Delta (CSNK1d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Casein Kinase 1 Delta (CSNK1d) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Casein Kinase 1 Delta (CSNK1d) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Casein Kinase 1 Delta (CSNK1d) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Casein Kinase 1 delta (CSNK1D) (Casein Kinase 1, delta (CSNK1D))

Autre désignation: Casein Kinase 1 Delta (CSNK1D Produits)

Synonymes: ASPS Kit ELISA, CKIdelta Kit ELISA, FASPS2 Kit ELISA, HCKID Kit ELISA, 1200006A05Rik Kit ELISA, AA409348 Kit ELISA, D930010H05Rik Kit ELISA, CKI-delta Kit ELISA, CKId Kit ELISA, csnk1d Kit ELISA, wu:fb69b03 Kit ELISA, wu:fj97a05 Kit ELISA, zgc:77627 Kit ELISA, CSNK1D Kit ELISA, ckIdelta Kit ELISA, Csnk1d Kit ELISA, csnk1dl Kit ELISA, wu:fb50c04 Kit ELISA, zgc:63971 Kit ELISA, casein kinase 1 delta Kit ELISA, casein kinase 1, delta Kit ELISA, casein kinase 1, delta b Kit ELISA, casein kinase 1 delta S homeolog Kit ELISA, casein kinase 1, delta a Kit ELISA, CSNK1D Kit ELISA, Csnk1d Kit ELISA, csnk1d Kit ELISA, csnk1db Kit ELISA, Bm1_37175 Kit ELISA, csnk1d.S Kit ELISA, csnk1da Kit ELISA

Sujet:

Gene Name: Casein Kinase 1 Delta

Gene Aliases: HCKID, Tau-protein kinase CSNK1D, Casein kinase I isoform delta

ID gène: 1453

UniProt: P48730

Pathways: Signalisation Hedgehog, M Phase