CTNNA1 Kit ELISA

N° du produit ABIN5652678

Détail du produit CTNNA1 Kit ELISA

Antigène: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Catenin Alpha 1 (CTNNa1). No significant cross-reactivity or interference between Catenin Alpha 1 (CTNNa1) and analogues was observed.

Sensibilité: 0.054 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catenin Alpha 1 (CTNNa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catenin Alpha 1 (CTNNa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catenin Alpha 1 (CTNNa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catenin Alpha 1 (CTNNa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Catenin Alpha 1 (CTNNa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Autre désignation: Catenin Alpha 1 (CTNNA1 Produits)

Synonymes: CAP102 Kit ELISA, 2010010M04Rik Kit ELISA, AA517462 Kit ELISA, AI988031 Kit ELISA, Catna1 Kit ELISA, CG17947 Kit ELISA, CT39986 Kit ELISA, Dalpha-Cat Kit ELISA, Dalpha-cat Kit ELISA, DalphaC Kit ELISA, Dalphacat Kit ELISA, Dmcatp Kit ELISA, Dmel\\CG17947 Kit ELISA, a-Cat Kit ELISA, a-cat Kit ELISA, alpha-CAT Kit ELISA, alpha-cat Kit ELISA, alpha-catenin Kit ELISA, alpha-ctn Kit ELISA, alphaCAT Kit ELISA, alphaCat Kit ELISA, alphacat Kit ELISA, Alpha-Cat Kit ELISA, dgri_GLEANR_3829 Kit ELISA, DgriGH19548 Kit ELISA, GH19548 Kit ELISA, ctnna Kit ELISA, cap102 Kit ELISA, catna1 Kit ELISA, ctnna1 Kit ELISA, catenin alpha 1 Kit ELISA, catenin (cadherin associated protein), alpha 1 Kit ELISA, alpha Catenin Kit ELISA, catenin alpha-1 Kit ELISA, catenin (cadherin-associated protein), alpha 1 Kit ELISA, catenin alpha 1 S homeolog Kit ELISA, CTNNA1 Kit ELISA, Ctnna1 Kit ELISA, alpha-Cat Kit ELISA, Dgri\alpha-Cat Kit ELISA, Tsp_10127 Kit ELISA, ctnna1 Kit ELISA, ctnna1.S Kit ELISA

Sujet:

Gene Name: Catenin Alpha 1

Gene Aliases: CTNN-A1, aCat, CAP102, Cadherin Associated Protein Alpha 1, Alpha E-catenin, Renal carcinoma antigen NY-REN-13

ID gène: 1495

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location