Cathepsin K Kit ELISA
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- Antigène Voir toutes Cathepsin K (CTSK) Kits ELISA
- Cathepsin K (CTSK)
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 12.5 pg/mL - 800 pg/mL
- Seuil minimal de détection
- 12.5 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Cathepsin K (CTSK). No significant cross-reactivity or interference between Cathepsin K (CTSK) and analogues was observed.
- Sensibilité
- 5.5 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin K (CTSK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin K (CTSK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin K (CTSK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin K (CTSK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin K (CTSK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin K (CTSK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes Cathepsin K (CTSK) Kits ELISA
- Cathepsin K (CTSK)
- Autre désignation
- Cathepsin K (CTSK Produits)
- Synonymes
- CTS02 Kit ELISA, CTSO Kit ELISA, CTSO1 Kit ELISA, CTSO2 Kit ELISA, PKND Kit ELISA, PYCD Kit ELISA, AI323530 Kit ELISA, MMS10-Q Kit ELISA, Ms10q Kit ELISA, catK Kit ELISA, CTSK Kit ELISA, wu:fa95f03 Kit ELISA, wu:fb08b05 Kit ELISA, zgc:110367 Kit ELISA, cts02 Kit ELISA, ctso Kit ELISA, ctso1 Kit ELISA, ctso2 Kit ELISA, pknd Kit ELISA, pycd Kit ELISA, OC-2 Kit ELISA, catk Kit ELISA, cathepsin K Kit ELISA, cathepsin K L homeolog Kit ELISA, Cathepsin K Kit ELISA, CTSK Kit ELISA, Ctsk Kit ELISA, ctsk Kit ELISA, ctsk.L Kit ELISA, catk Kit ELISA
- Sujet
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Gene Name: Cathepsin K
Gene Aliases: CTS-K, CTS02, CTSO, CTSO1, CTSO2, PKND, PYCD, Pycnodysostosis, Cathepsin O, Cathepsin X
- ID gène
- 13038
- UniProt
- P55097
- Pathways
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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