CADM1 Kit ELISA

N° du produit ABIN5652756

Détail du produit CADM1 Kit ELISA

Antigène: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cell Adhesion Molecule 1 (CADM1). No significant cross-reactivity or interference between Cell Adhesion Molecule 1 (CADM1) and analogues was observed.

Sensibilité: 0.071 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Adhesion Molecule 1 (CADM1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cell Adhesion Molecule 1 (CADM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Adhesion Molecule 1 (CADM1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cell Adhesion Molecule 1 (CADM1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cell Adhesion Molecule 1 (CADM1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cell Adhesion Molecule 1 (CADM1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CADM1 (Cell Adhesion Molecule 1 (CADM1))

Autre désignation: Cell Adhesion Molecule 1 (CADM1 Produits)

Synonymes: BL2 Kit ELISA, IGSF4 Kit ELISA, IGSF4A Kit ELISA, NECL2 Kit ELISA, Necl-2 Kit ELISA, RA175 Kit ELISA, ST17 Kit ELISA, SYNCAM Kit ELISA, TSLC1 Kit ELISA, sTSLC-1 Kit ELISA, sgIGSF Kit ELISA, synCAM1 Kit ELISA, bl2 Kit ELISA, igsf4 Kit ELISA, igsf4a Kit ELISA, necl-2 Kit ELISA, necl2 Kit ELISA, ra175 Kit ELISA, sgigsf Kit ELISA, st17 Kit ELISA, stslc-1 Kit ELISA, syncam Kit ELISA, syncam1 Kit ELISA, tslc1 Kit ELISA, xnecl2 Kit ELISA, 2900073G06Rik Kit ELISA, 3100001I08Rik Kit ELISA, AI987920 Kit ELISA, Bl2 Kit ELISA, Igsf4 Kit ELISA, Igsf4a Kit ELISA, Necl2 Kit ELISA, RA175A Kit ELISA, RA175B Kit ELISA, RA175C Kit ELISA, RA175N Kit ELISA, SgIGSF Kit ELISA, SynCam Kit ELISA, Tslc1 Kit ELISA, SynCAM1 Kit ELISA, cell adhesion molecule 1 Kit ELISA, cell adhesion molecule 1 L homeolog Kit ELISA, CADM1 Kit ELISA, cadm1.L Kit ELISA, Cadm1 Kit ELISA

Sujet:

Gene Name: Cell Adhesion Molecule 1

Gene Aliases: BL2, IGSF4, IGSF4A, NECL2, RA175, ST17, SYNCAM, TSLC1, sTSLC-1, synCAM1, Tumor Suppressor In Lung Cancer 1, Immunoglobulin Superfamily,Member 4, Nectin-Like 2

ID gène: 23705

UniProt: Q9BY67

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Activated T Cell Proliferation