CDC42 Kit ELISA

N° du produit ABIN5652760

Détail du produit CDC42 Kit ELISA

Antigène: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cell Division Cycle Protein 42 (CDC42). No significant cross-reactivity or interference between Cell Division Cycle Protein 42 (CDC42) and analogues was observed.

Sensibilité: 0.064 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Division Cycle Protein 42 (CDC42). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cell Division Cycle Protein 42 (CDC42). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Division Cycle Protein 42 (CDC42), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cell Division Cycle Protein 42 (CDC42) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cell Division Cycle Protein 42 (CDC42) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cell Division Cycle Protein 42 (CDC42) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Autre désignation: Cell Division Cycle Protein 42 (CDC42 Produits)

Synonymes: CDC42Hs Kit ELISA, G25K Kit ELISA, AI747189 Kit ELISA, AU018915 Kit ELISA, cdc42a Kit ELISA, wu:fb07d08 Kit ELISA, wu:fj62g06 Kit ELISA, zgc:63918 Kit ELISA, Xcdc42 Kit ELISA, cdc42hs Kit ELISA, g25k Kit ELISA, CDC42 Kit ELISA, CG12530 Kit ELISA, Cdc42Dm Kit ELISA, D-CDC42 Kit ELISA, D-Cdc42 Kit ELISA, D-cdc42 Kit ELISA, DCDC42 Kit ELISA, DCdc42 Kit ELISA, Dcdc42 Kit ELISA, Dm Cdc42 Kit ELISA, DmCDC42 Kit ELISA, Dmcdc42 Kit ELISA, Dmel\\CG12530 Kit ELISA, cdc42 Kit ELISA, cell division cycle 42 Kit ELISA, Cell division control protein 42 homolog Kit ELISA, cell division control protein 42 homolog Kit ELISA, cell division cycle 42 L homeolog Kit ELISA, CG12530 gene product from transcript CG12530-RC Kit ELISA, Rho family GTPase Kit ELISA, CDC42 Kit ELISA, Cdc42 Kit ELISA, cdc42 Kit ELISA, LOC100636735 Kit ELISA, LOC100637699 Kit ELISA, LOC100638834 Kit ELISA, cdc42.L Kit ELISA

Sujet:

Gene Name: Cell Division Cycle Protein 42

Gene Aliases: CDC42Hs, G25K, G25K GTP-binding protein

ID gène: 998

UniProt: P60953

Pathways: Signalisation MAPK, Dynamique des Microtubules, Signalisation RTK, Signalisation WNT, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling