CRABP2 Kit ELISA

N° du produit ABIN5652763

Détail du produit CRABP2 Kit ELISA

Antigène: CRABP2 (Cellular Retinoic Acid Binding Protein 2 (CRABP2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cellular Retinoic Acid Binding Protein 2 (CRABP2). No significant cross-reactivity or interference between Cellular Retinoic Acid Binding Protein 2 (CRABP2) and analogues was observed.

Sensibilité: 0.116 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cellular Retinoic Acid Binding Protein 2 (CRABP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cellular Retinoic Acid Binding Protein 2 (CRABP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cellular Retinoic Acid Binding Protein 2 (CRABP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cellular Retinoic Acid Binding Protein 2 (CRABP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cellular Retinoic Acid Binding Protein 2 (CRABP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cellular Retinoic Acid Binding Protein 2 (CRABP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CRABP2 (Cellular Retinoic Acid Binding Protein 2 (CRABP2))

Autre désignation: Cellular Retinoic Acid Binding Protein 2 (CRABP2 Produits)

Synonymes: crabp Kit ELISA, crabp2-A Kit ELISA, xCRABP Kit ELISA, xCRABP-b Kit ELISA, cb432 Kit ELISA, cb434 Kit ELISA, crabp2 Kit ELISA, sb:cb434 Kit ELISA, wu:fc51a11 Kit ELISA, zgc:110496 Kit ELISA, CRABP2 Kit ELISA, rbp6 Kit ELISA, MGC89469 Kit ELISA, crabp-ii Kit ELISA, CRABP-II Kit ELISA, RBP6 Kit ELISA, AI893628 Kit ELISA, Crabp-2 Kit ELISA, CrabpII Kit ELISA, im:7136989 Kit ELISA, cellular retinoic acid binding protein 2 L homeolog Kit ELISA, cellular retinoic acid binding protein 2, a Kit ELISA, cellular retinoic acid binding protein 2 Kit ELISA, cellular retinoic acid binding protein II Kit ELISA, cellular retinoic acid binding protein 2, b Kit ELISA, crabp2.L Kit ELISA, crabp2a Kit ELISA, CRABP2 Kit ELISA, crabp2 Kit ELISA, Crabp2 Kit ELISA, crabp2b Kit ELISA

Sujet:

Gene Name: Cellular Retinoic Acid Binding Protein 2

Gene Aliases: CRABP-II, RBP6

ID gène: 1382

UniProt: P29373