CERK Kit ELISA

N° du produit ABIN5652767

Détail du produit CERK Kit ELISA

Antigène: CERK (Ceramide Kinase (CERK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Seuil minimal de détection: 3.12 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ceramide Kinase (CERK). No significant cross-reactivity or interference between Ceramide Kinase (CERK) and analogues was observed.

Sensibilité: 1.14 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ceramide Kinase (CERK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ceramide Kinase (CERK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ceramide Kinase (CERK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ceramide Kinase (CERK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ceramide Kinase (CERK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ceramide Kinase (CERK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CERK (Ceramide Kinase (CERK))

Autre désignation: Ceramide Kinase (CERK Produits)

Synonymes: AI848610 Kit ELISA, D330016D08Rik Kit ELISA, mKIAA1646 Kit ELISA, LK4 Kit ELISA, dA59H18.2 Kit ELISA, dA59H18.3 Kit ELISA, hCERK Kit ELISA, CERK Kit ELISA, si:ch211-162i19.1 Kit ELISA, MGC81777 Kit ELISA, CG16708 Kit ELISA, DCERK Kit ELISA, Dcerk Kit ELISA, Dmel\\CG16708 Kit ELISA, dcerk Kit ELISA, ceramide kinase Kit ELISA, ceramide kinase S homeolog Kit ELISA, Ceramide kinase Kit ELISA, Cerk Kit ELISA, CERK Kit ELISA, cerk Kit ELISA, cerk.S Kit ELISA, CpipJ_CPIJ003342 Kit ELISA, LOAG_11245 Kit ELISA, LOC5579301 Kit ELISA, Bm1_50005 Kit ELISA

Sujet:

Gene Name: Ceramide Kinase

Gene Aliases: LK4, hCERK, Lipid kinase 4, Acylsphingosine kinase