CDNF Kit ELISA

N° du produit ABIN5652776

Détail du produit CDNF Kit ELISA

Antigène: CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cerebral Dopamine Neurotrophic Factor (CDNF). No significant cross-reactivity or interference between Cerebral Dopamine Neurotrophic Factor (CDNF) and analogues was observed.

Sensibilité: 0.056 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cerebral Dopamine Neurotrophic Factor (CDNF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cerebral Dopamine Neurotrophic Factor (CDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cerebral Dopamine Neurotrophic Factor (CDNF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cerebral Dopamine Neurotrophic Factor (CDNF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cerebral Dopamine Neurotrophic Factor (CDNF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cerebral Dopamine Neurotrophic Factor (CDNF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CDNF (Cerebral Dopamine Neurotrophic Factor (CDNF))

Autre désignation: Cerebral Dopamine Neurotrophic Factor (CDNF Produits)

Synonymes: ARMETL1 Kit ELISA, 9330140G23 Kit ELISA, Armetl1 Kit ELISA, cerebral dopamine neurotrophic factor Kit ELISA, CDNF Kit ELISA, Cdnf Kit ELISA

Sujet:

Gene Name: Cerebral Dopamine Neurotrophic Factor

Gene Aliases: ARMETL1, ARMET-like protein 1, Conserved Dopamine Neurotrophic Factor, Arginine-Rich,Mutated In Early Stage Tumors-Like 1

ID gène: 361276

UniProt: P0C5I0