Galectin 10 Kit ELISA

N° du produit ABIN5652787

Détail du produit Galectin 10 Kit ELISA

Antigène: Galectin 10 (CLC) (Charcot-Leyden Crystal Protein (CLC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Charcot Leyden Crystal Protein (CLC). No significant cross-reactivity or interference between Charcot Leyden Crystal Protein (CLC) and analogues was observed.

Sensibilité: 0.126 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Charcot Leyden Crystal Protein (CLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Charcot Leyden Crystal Protein (CLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Charcot Leyden Crystal Protein (CLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Charcot Leyden Crystal Protein (CLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Charcot Leyden Crystal Protein (CLC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Charcot Leyden Crystal Protein (CLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Galectin 10 (CLC) (Charcot-Leyden Crystal Protein (CLC))

Autre désignation: Charcot Leyden Crystal Protein (CLC Produits)

Synonymes: GAL10 Kit ELISA, Gal-10 Kit ELISA, LGALS10 Kit ELISA, LGALS10A Kit ELISA, LPPL_HUMAN Kit ELISA, Clcp Kit ELISA, gal-10 Kit ELISA, Bsf3 Kit ELISA, CLC Kit ELISA, GAL 10 Kit ELISA, Gal-8 Kit ELISA, Gal8 Kit ELISA, Charcot-Leyden crystal galectin Kit ELISA, Charcot-Leyden crystal protein Kit ELISA, cardiotrophin-like cytokine factor 1 Kit ELISA, avian beta-defensin 10 Kit ELISA, CLC Kit ELISA, Clc Kit ELISA, Clcf1 Kit ELISA, AvBD10 Kit ELISA

Sujet:

Gene Name: Charcot Leyden Crystal Protein

Gene Aliases: LGALS10, LPPL, Eosinophil Lysophospholipase, Lysolecithin Acylhydrolase, Galectin 10

ID gène: 1178

UniProt: Q05315

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response