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Galectin 10 Kit ELISA

CLC Reactivité: Souris Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN5652788
  • Antigène Voir toutes Galectin 10 (CLC) Kits ELISA
    Galectin 10 (CLC) (Charcot-Leyden Crystal Protein (CLC))
    Reactivité
    • 7
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Charcot Leyden Crystal Protein (CLC). No significant cross-reactivity or interference between Charcot Leyden Crystal Protein (CLC) and analogues was observed.
    Sensibilité
    0.054 ng/mL
    Top Product
    Discover our top product CLC Kit ELISA
  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Charcot Leyden Crystal Protein (CLC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Charcot Leyden Crystal Protein (CLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Charcot Leyden Crystal Protein (CLC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Charcot Leyden Crystal Protein (CLC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Charcot Leyden Crystal Protein (CLC) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Charcot Leyden Crystal Protein (CLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes Galectin 10 (CLC) Kits ELISA
    Galectin 10 (CLC) (Charcot-Leyden Crystal Protein (CLC))
    Autre désignation
    Charcot Leyden Crystal Protein (CLC Produits)
    Synonymes
    GAL10 Kit ELISA, Gal-10 Kit ELISA, LGALS10 Kit ELISA, LGALS10A Kit ELISA, LPPL_HUMAN Kit ELISA, Clcp Kit ELISA, gal-10 Kit ELISA, Bsf3 Kit ELISA, CLC Kit ELISA, GAL 10 Kit ELISA, Gal-8 Kit ELISA, Gal8 Kit ELISA, Charcot-Leyden crystal galectin Kit ELISA, Charcot-Leyden crystal protein Kit ELISA, cardiotrophin-like cytokine factor 1 Kit ELISA, avian beta-defensin 10 Kit ELISA, CLC Kit ELISA, Clc Kit ELISA, Clcf1 Kit ELISA, AvBD10 Kit ELISA
    Sujet

    Gene Name: Charcot Leyden Crystal Protein

    Gene Aliases: LGALS10, LPPL, Eosinophil Lysophospholipase, Lysolecithin Acylhydrolase, Galectin 10

    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response
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