CLOCK Kit ELISA

N° du produit ABIN5652968

Détail du produit CLOCK Kit ELISA

Antigène: CLOCK (Clock Homolog (Mouse) (CLOCK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Circadian Locomoter Output Cycles Protein Kaput (CLOCK). No significant cross-reactivity or interference between Circadian Locomoter Output Cycles Protein Kaput (CLOCK) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Circadian Locomoter Output Cycles Protein Kaput (CLOCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Circadian Locomoter Output Cycles Protein Kaput (CLOCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Circadian Locomoter Output Cycles Protein Kaput (CLOCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Circadian Locomoter Output Cycles Protein Kaput (CLOCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Circadian Locomoter Output Cycles Protein Kaput (CLOCK) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Circadian Locomoter Output Cycles Protein Kaput (CLOCK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CLOCK (Clock Homolog (Mouse) (CLOCK))

Autre désignation: Circadian Locomoter Output Cycles Protein Kaput (CLOCK Produits)

Synonymes: CG7391 Kit ELISA, CLK Kit ELISA, CLOCK Kit ELISA, Dmel\\CG7391 Kit ELISA, Jerk Kit ELISA, Jrk Kit ELISA, PAS1 Kit ELISA, bHLHe10 Kit ELISA, clk Kit ELISA, clock Kit ELISA, dCLK Kit ELISA, dCLK/JRK Kit ELISA, dCLOCK Kit ELISA, dClck Kit ELISA, dClk Kit ELISA, dClock Kit ELISA, dclk Kit ELISA, jrk Kit ELISA, Xclk Kit ELISA, XClock Kit ELISA, zCLOCK1 Kit ELISA, zfCLOCK1 Kit ELISA, 5330400M04Rik Kit ELISA, KAT13D Kit ELISA, bHLHe8 Kit ELISA, mKIAA0334 Kit ELISA, Clock Kit ELISA, clock circadian regulator Kit ELISA, clock homolog (mouse) Kit ELISA, clock circadian regulator a Kit ELISA, circadian locomotor output cycles kaput Kit ELISA, clock circadian regulator L homeolog Kit ELISA, Clk Kit ELISA, CLOCK Kit ELISA, clock Kit ELISA, clocka Kit ELISA, Clock Kit ELISA, clock.L Kit ELISA

Sujet:

Gene Name: Circadian Locomoter Output Cycles Protein Kaput

Gene Aliases: KAT13D, bHLHe8, Class E basic helix-loop-helix protein 8

ID gène: 9575

UniProt: O15516

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism