CD3D Kit ELISA

N° du produit ABIN5653017

Détail du produit CD3D Kit ELISA

Antigène: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 3d (CD3d). No significant cross-reactivity or interference between Cluster Of Differentiation 3d (CD3d) and analogues was observed.

Sensibilité: 0.114 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 3d (CD3d). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 3d (CD3d). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 3d (CD3d), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 3d (CD3d) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 3d (CD3d) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 3d (CD3d) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Autre désignation: Cluster Of Differentiation 3d (CD3D Produits)

Synonymes: CD3-DELTA Kit ELISA, T3D Kit ELISA, CD3 Kit ELISA, T3d Kit ELISA, CD3d molecule Kit ELISA, CD3 antigen, delta polypeptide Kit ELISA, CD3D Kit ELISA, Cd3d Kit ELISA

Sujet:

Gene Name: Cluster Of Differentiation 3d

Gene Aliases: CD3-DELTA, T3D, T-Dell Surface Glycoprotein CD3 Delta Chain, T-cell receptor T3 delta chain

ID gène: 915

UniProt: P04234

Pathways: TCR Signaling, CXCR4-mediated Signaling Events