CD8 alpha Kit ELISA

N° du produit ABIN5653029

Détail du produit CD8 alpha Kit ELISA

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 8a (CD8a). No significant cross-reactivity or interference between Cluster Of Differentiation 8a (CD8a) and analogues was observed.

Sensibilité: 0.57 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 8a (CD8a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 8a (CD8a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 8a (CD8a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cluster Of Differentiation 8a (CD8a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 8a (CD8a) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 8a (CD8a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: Cluster Of Differentiation 8a (CD8A Produits)

Synonymes: cd8 Kit ELISA, LOC100136450 Kit ELISA, BB154331 Kit ELISA, Ly-2 Kit ELISA, Ly-35 Kit ELISA, Ly-B Kit ELISA, Lyt-2 Kit ELISA, zgc:136643 Kit ELISA, CD8 Kit ELISA, Leu2 Kit ELISA, MAL Kit ELISA, p32 Kit ELISA, RHACD8-4 Kit ELISA, CD8a molecule Kit ELISA, uncharacterized LOC100125537 Kit ELISA, CD8 alpha Kit ELISA, CD8 antigen, alpha chain Kit ELISA, T-cell surface glycoprotein CD8 alpha chain Kit ELISA, Cd8a Kit ELISA, LOC100125537 Kit ELISA, LOC100136450 Kit ELISA, CD8A Kit ELISA, cd8a Kit ELISA, LOC100126556 Kit ELISA

Sujet:

Gene Name: Cluster Of Differentiation 8a

Gene Aliases: p32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

ID gène: 925

UniProt: P01732

Pathways: TCR Signaling