M-CSF/CSF1 Kit ELISA
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- Antigène Voir toutes M-CSF/CSF1 (CSF1) Kits ELISA
- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 6.25 pg/mL - 400 pg/mL
- Seuil minimal de détection
- 6.25 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Colony Stimulating Factor 1, Macrophage (MCSF). No significant cross-reactivity or interference between Colony Stimulating Factor 1, Macrophage (MCSF) and analogues was observed.
- Sensibilité
- 2.37 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 1, Macrophage (MCSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 1, Macrophage (MCSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 1, Macrophage (MCSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Colony Stimulating Factor 1, Macrophage (MCSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 1, Macrophage (MCSF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 1, Macrophage (MCSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes M-CSF/CSF1 (CSF1) Kits ELISA
- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
- Autre désignation
- Colony Stimulating Factor 1, Macrophage (CSF1 Produits)
- Synonymes
- CSF-1 Kit ELISA, MCSF Kit ELISA, C87615 Kit ELISA, Csfm Kit ELISA, op Kit ELISA, csf1-1 Kit ELISA, zgc:172186 Kit ELISA, CSF1 Kit ELISA, csf1-2 Kit ELISA, zgc:158436 Kit ELISA, colony stimulating factor 1 Kit ELISA, colony stimulating factor 1 (macrophage) Kit ELISA, colony stimulating factor 1a (macrophage) Kit ELISA, macrophage colony-stimulating factor 1 Kit ELISA, macrophage colony stimulating factor Kit ELISA, colony stimulating factor 1b (macrophage) Kit ELISA, CSF1 Kit ELISA, Csf1 Kit ELISA, csf1a Kit ELISA, LOC396599 Kit ELISA, LOC100860895 Kit ELISA, csf1b Kit ELISA
- Sujet
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Gene Name: Colony Stimulating Factor 1, Macrophage
Gene Aliases: CSF1, M-CSF, Lanimostim, Processed macrophage colony-stimulating factor 1
- ID gène
- 12977
- UniProt
- P07141
- Pathways
- Signalisation RTK
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