Complement C4 Kit ELISA
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- Antigène Voir toutes Complement C4 (C4) Kits ELISA
- Complement C4 (C4) (Complement 4 (C4))
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 6.25 ng/mL - 400 ng/mL
- Seuil minimal de détection
- 6.25 ng/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Complement Component 4 (C4). No significant cross-reactivity or interference between Complement Component 4 (C4) and analogues was observed.
- Sensibilité
- 2.74 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 4 (C4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 4 (C4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 4 (C4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Component 4 (C4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 4 (C4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 4 (C4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes Complement C4 (C4) Kits ELISA
- Complement C4 (C4) (Complement 4 (C4))
- Autre désignation
- Complement Component 4 (C4 Produits)
- Synonymes
- C4B1 Kit ELISA, C4B12 Kit ELISA, C4B2 Kit ELISA, C4B3 Kit ELISA, C4B5 Kit ELISA, C4BD Kit ELISA, C4B_2 Kit ELISA, C4F Kit ELISA, CH Kit ELISA, CO4 Kit ELISA, CPAMD3 Kit ELISA, C4 Kit ELISA, C4A2 Kit ELISA, C4A3 Kit ELISA, C4A4 Kit ELISA, C4A6 Kit ELISA, C4AD Kit ELISA, C4S Kit ELISA, CPAMD2 Kit ELISA, RG Kit ELISA, C4-1 Kit ELISA, C4b Kit ELISA, C4-2 Kit ELISA, Slp Kit ELISA, Ss Kit ELISA, complement C4B (Chido blood group) Kit ELISA, complement C4A (Rodgers blood group) Kit ELISA, complement component 4A (Rodgers blood group) Kit ELISA, complement 4 Kit ELISA, complement component 4A Kit ELISA, complement component 4B (Chido blood group) Kit ELISA, C4B Kit ELISA, C4A Kit ELISA, C4a Kit ELISA, C4 Kit ELISA, C4b Kit ELISA
- Sujet
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Gene Name: Complement Component 4
Gene Aliases: C4BP-a, Complement Component 4 Binding Protein,Alpha
- Pathways
- Système du Complément
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