CD35 Kit ELISA

N° du produit ABIN5653283

Détail du produit CD35 Kit ELISA

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Complement Receptor 1, Erythrocyte (CR1). No significant cross-reactivity or interference between Complement Receptor 1, Erythrocyte (CR1) and analogues was observed.

Sensibilité: 12.9 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Receptor 1, Erythrocyte (CR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Receptor 1, Erythrocyte (CR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Receptor 1, Erythrocyte (CR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Receptor 1, Erythrocyte (CR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Receptor 1, Erythrocyte (CR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Receptor 1, Erythrocyte (CR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Autre désignation: Complement Receptor 1, Erythrocyte (CR1 Produits)

Synonymes: C3BR Kit ELISA, C4BR Kit ELISA, CD35 Kit ELISA, KN Kit ELISA, C3DR Kit ELISA, CD21 Kit ELISA, Cr-1 Kit ELISA, Cr-2 Kit ELISA, Cr1 Kit ELISA, CR1-L Kit ELISA, CR2 Kit ELISA, CR1 Kit ELISA, complement C3b/C4b receptor 1 (Knops blood group) Kit ELISA, complement receptor 2 Kit ELISA, complement component (3d/Epstein Barr virus) receptor 2 Kit ELISA, complement receptor type 1 Kit ELISA, CR1 Kit ELISA, Cr2 Kit ELISA, CR2 Kit ELISA, LOC718120 Kit ELISA

Sujet:

Gene Name: Complement Receptor 1, Erythrocyte

Gene Aliases: CD35, C3BR, KN, C3b/C4b Receptor,Including Knops Blood Group System, Immune Adherence Receptor

ID gène: 1378

UniProt: P17927

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity