CRHBP Kit ELISA

N° du produit ABIN5653342

Détail du produit CRHBP Kit ELISA

Antigène: CRHBP (Corticotropin Releasing Hormone Binding Protein (CRHBP))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Corticotropin Releasing Hormone Binding Protein (CRHBP). No significant cross-reactivity or interference between Corticotropin Releasing Hormone Binding Protein (CRHBP) and analogues was observed.

Sensibilité: 6.3 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Corticotropin Releasing Hormone Binding Protein (CRHBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Corticotropin Releasing Hormone Binding Protein (CRHBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Corticotropin Releasing Hormone Binding Protein (CRHBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Corticotropin Releasing Hormone Binding Protein (CRHBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticotropin Releasing Hormone Binding Protein (CRHBP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticotropin Releasing Hormone Binding Protein (CRHBP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CRHBP (Corticotropin Releasing Hormone Binding Protein (CRHBP))

Autre désignation: Corticotropin Releasing Hormone Binding Protein (CRHBP Produits)

Synonymes: crh-BP Kit ELISA, GB14094 Kit ELISA, zgc:91905 Kit ELISA, CRHBP Kit ELISA, CRH-BP Kit ELISA, CRF-BP Kit ELISA, CRFBP Kit ELISA, Crfbp Kit ELISA, crhbp-A Kit ELISA, gene E Kit ELISA, corticotropin releasing hormone binding protein Kit ELISA, corticotropin releasing hormone binding protein S homeolog Kit ELISA, CRHBP Kit ELISA, crhbp Kit ELISA, crh-BP Kit ELISA, Crhbp Kit ELISA, crhbp.S Kit ELISA

Sujet:

Gene Name: Corticotropin Releasing Hormone Binding Protein

Gene Aliases: CRF-BP, CRFBP, Corticotropin-Releasing Factor-Binding Protein

UniProt: P24388

Pathways: Negative Regulation of Hormone Secretion