CRY1 Kit ELISA

N° du produit ABIN5653385

Détail du produit CRY1 Kit ELISA

Antigène: CRY1 (Cryptochrome 1 (Photolyase-Like) (CRY1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cryptochrome 1 (CRY1). No significant cross-reactivity or interference between Cryptochrome 1 (CRY1) and analogues was observed.

Sensibilité: 0.129 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cryptochrome 1 (CRY1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cryptochrome 1 (CRY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cryptochrome 1 (CRY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cryptochrome 1 (CRY1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cryptochrome 1 (CRY1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cryptochrome 1 (CRY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CRY1 (Cryptochrome 1 (Photolyase-Like) (CRY1))

Autre désignation: Cryptochrome 1 (CRY1 Produits)

Synonymes: cry1-A Kit ELISA, CRY1 Kit ELISA, cry2 Kit ELISA, phll1 Kit ELISA, xCRY1 Kit ELISA, PHLL1 Kit ELISA, AU020726 Kit ELISA, AU021000 Kit ELISA, Phll1 Kit ELISA, ATCRY1 Kit ELISA, BLU1 Kit ELISA, BLUE LIGHT UNINHIBITED 1 Kit ELISA, CRYPTOCHROME 1 APOPROTEIN (BLUE LIGHT PHOTORECEPTOR Kit ELISA, ELONGATED HYPOCOTYL 4 Kit ELISA, HY4 Kit ELISA, OOP2 Kit ELISA, OUT OF PHASE 2 Kit ELISA, T3H13.14 Kit ELISA, T3H13_14 Kit ELISA, cryptochrome 1 Kit ELISA, cryptochrome circadian clock 1 L homeolog Kit ELISA, cryptochrome circadian regulator 1 Kit ELISA, cryptochrome circadian clock 1 Kit ELISA, Cryptochrome-1 Kit ELISA, cryptochrome 1 Kit ELISA, cryptochrome 1 (photolyase-like) Kit ELISA, cry1.L Kit ELISA, CRY1 Kit ELISA, cry1 Kit ELISA, siu50817b Kit ELISA, Cry1 Kit ELISA

Sujet:

Gene Name: Cryptochrome 1

Gene Aliases: PHLL1, Photolyase-like 1

ID gène: 1407

UniProt: Q16526

Pathways: Response to Water Deprivation, Proton Transport