Cyclin D2 Kit ELISA
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- Antigène Voir toutes Cyclin D2 (CCND2) Kits ELISA
- Cyclin D2 (CCND2)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.62 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 15.62 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Cyclin D2 (CCND2). No significant cross-reactivity or interference between Cyclin D2 (CCND2) and analogues was observed.
- Sensibilité
- 5.9 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin D2 (CCND2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclin D2 (CCND2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclin D2 (CCND2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclin D2 (CCND2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclin D2 (CCND2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclin D2 (CCND2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes Cyclin D2 (CCND2) Kits ELISA
- Cyclin D2 (CCND2)
- Autre désignation
- Cyclin D2 (CCND2 Produits)
- Synonymes
- si:ch211-240j22.2 Kit ELISA, ccnd2 Kit ELISA, CCND2 Kit ELISA, kiak0002 Kit ELISA, zgc:162280 Kit ELISA, LOC100224096 Kit ELISA, KIAK0002 Kit ELISA, BF642806 Kit ELISA, C86853 Kit ELISA, Vin-1 Kit ELISA, Vin1 Kit ELISA, cD2 Kit ELISA, 2600016F06Rik Kit ELISA, AI256817 Kit ELISA, cyclin D2, b Kit ELISA, cyclin D2 S homeolog Kit ELISA, cyclin D2 Kit ELISA, G1/S-specific cyclin-D2 Kit ELISA, cyclin D2, a Kit ELISA, cyclin D2 L homeolog Kit ELISA, ccnd2b Kit ELISA, ccnd2.S Kit ELISA, CCND2 Kit ELISA, ccnd2 Kit ELISA, ccnd2a Kit ELISA, Ccnd2 Kit ELISA, ccnd2.L Kit ELISA
- Sujet
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Gene Name: Cyclin D2
Gene Aliases: CCN-D2, G1/S-Specific Cyclin-D2
- ID gène
- 894
- UniProt
- P30279
- Pathways
- Cycle Cellulaire, Mitotic G1-G1/S Phases
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