CSRP1 Kit ELISA

N° du produit ABIN5653462

Détail du produit CSRP1 Kit ELISA

Antigène: CSRP1 (Cysteine and Glycine-Rich Protein 1 (CSRP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cysteine And Glycine Rich Protein 1 (CSRP1). No significant cross-reactivity or interference between Cysteine And Glycine Rich Protein 1 (CSRP1) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine And Glycine Rich Protein 1 (CSRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine And Glycine Rich Protein 1 (CSRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine And Glycine Rich Protein 1 (CSRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine And Glycine Rich Protein 1 (CSRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine And Glycine Rich Protein 1 (CSRP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine And Glycine Rich Protein 1 (CSRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CSRP1 (Cysteine and Glycine-Rich Protein 1 (CSRP1))

Autre désignation: Cysteine And Glycine Rich Protein 1 (CSRP1 Produits)

Synonymes: CRP Kit ELISA, CRP1 Kit ELISA, CSRP Kit ELISA, CYRP Kit ELISA, D1S181E Kit ELISA, AA408841 Kit ELISA, AA959891 Kit ELISA, AW545626 Kit ELISA, Csrp Kit ELISA, cb796 Kit ELISA, csrp1 Kit ELISA, zgc:77332 Kit ELISA, MGC85577 Kit ELISA, MGC89932 Kit ELISA, CSRP1 Kit ELISA, zgc:158737 Kit ELISA, cysteine and glycine rich protein 1 Kit ELISA, cysteine and glycine-rich protein 1 Kit ELISA, cysteine and glycine-rich protein 1a Kit ELISA, cysteine and glycine rich protein 1 L homeolog Kit ELISA, cysteine and glycine-rich protein 1b Kit ELISA, Cysteine and glycine-rich protein 1 Kit ELISA, CSRP1 Kit ELISA, Csrp1 Kit ELISA, csrp1a Kit ELISA, csrp1.L Kit ELISA, csrp1 Kit ELISA, csrp1b Kit ELISA, LOC100304834 Kit ELISA, Tsp_01053 Kit ELISA

Sujet:

Gene Name: Cysteine And Glycine Rich Protein 1

Gene Aliases: CRP1, CRP, CSRP, CYRP, HEL-141, Cysteine-rich protein 1, Epididymis luminal protein 141

ID gène: 1465

UniProt: P21291