CRIP1 Kit ELISA

N° du produit ABIN5653464

Détail du produit CRIP1 Kit ELISA

Antigène: CRIP1 (Cysteine-Rich Protein 1 (Intestinal) (CRIP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Protein 1, Intestinal (CRIP1). No significant cross-reactivity or interference between Cysteine Rich Protein 1, Intestinal (CRIP1) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Protein 1, Intestinal (CRIP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Protein 1, Intestinal (CRIP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Protein 1, Intestinal (CRIP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich Protein 1, Intestinal (CRIP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Protein 1, Intestinal (CRIP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Protein 1, Intestinal (CRIP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CRIP1 (Cysteine-Rich Protein 1 (Intestinal) (CRIP1))

Autre désignation: Cysteine Rich Protein 1, Intestinal (CRIP1 Produits)

Synonymes: C11H2orf32 Kit ELISA, C2orf32 Kit ELISA, CRHP Kit ELISA, CRIP Kit ELISA, CRP-1 Kit ELISA, CRP1 Kit ELISA, Crip Kit ELISA, crhp Kit ELISA, crip Kit ELISA, crip1 Kit ELISA, crip1b Kit ELISA, crp1 Kit ELISA, crip1a Kit ELISA, zgc:103738 Kit ELISA, Crp-1 Kit ELISA, cannabinoid receptor interacting protein 1 Kit ELISA, cysteine rich protein 1 Kit ELISA, cysteine-rich protein 1 (intestinal) Kit ELISA, cysteine-rich protein 1 (intestinal) S homeolog Kit ELISA, cysteine-rich protein 1 (intestinal) L homeolog Kit ELISA, cysteine-rich protein 1 Kit ELISA, cysteine-rich protein 1 (intestinal) pseudogene Kit ELISA, CNRIP1 Kit ELISA, CRIP1 Kit ELISA, Crip1 Kit ELISA, crip1.S Kit ELISA, crip1.L Kit ELISA, crip1 Kit ELISA, LOC747660 Kit ELISA

Sujet:

Gene Name: Cysteine Rich Protein 1, Intestinal

Gene Aliases: CRP1, CRHP, CRIP, Cysteine-rich heart protein, Cysteine-rich intestinal protein

ID gène: 1396

UniProt: P50238