CYR61 Kit ELISA

N° du produit ABIN5653465

Détail du produit CYR61 Kit ELISA

Antigène: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). No significant cross-reactivity or interference between Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) and analogues was observed.

Sensibilité: 26.1 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Autre désignation: Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61 Produits)

Synonymes: CCN1 Kit ELISA, GIG1 Kit ELISA, IGFBP10 Kit ELISA, ccn1 Kit ELISA, gig1 Kit ELISA, Xcyr61 Kit ELISA, igfbp10 Kit ELISA, MGC69281 Kit ELISA, MGC69539 Kit ELISA, cyr61-l2 Kit ELISA, zgc:158158 Kit ELISA, CYR61 Kit ELISA, AI325051 Kit ELISA, Igfbp10 Kit ELISA, cysteine rich angiogenic inducer 61 Kit ELISA, cysteine-rich, angiogenic inducer, 61 Kit ELISA, cysteine-rich, angiogenic inducer, 61 like 2 Kit ELISA, cysteine rich protein 61 Kit ELISA, cysteine rich angiogenic inducer 61 S homeolog Kit ELISA, CYR61 Kit ELISA, Cyr61 Kit ELISA, cyr61 Kit ELISA, cyr61l2 Kit ELISA, cyr61.S Kit ELISA

Sujet:

Gene Name: Cysteine Rich Protein, Angiogenic Inducer 61

Gene Aliases: CCN1, GIG1, IGFBP10, CCN family member 1, Insulin-like growth factor-binding protein 10

ID gène: 3491

UniProt: O00622

Pathways: Positive Regulation of Endopeptidase Activity, Growth Factor Binding