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CRISP3 Kit ELISA

CRISP3 Reactivité: Humain Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Saliva, Serum, Sweat
N° du produit ABIN5653468
  • Antigène Voir toutes CRISP3 Kits ELISA
    CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.94 ng/mL - 60 ng/mL
    Seuil minimal de détection
    0.94 ng/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Saliva, Serum, Sweat
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Secretory Protein 3 (CRISP3). No significant cross-reactivity or interference between Cysteine Rich Secretory Protein 3 (CRISP3) and analogues was observed.
    Sensibilité
    0.43 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Secretory Protein 3 (CRISP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Secretory Protein 3 (CRISP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Secretory Protein 3 (CRISP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich Secretory Protein 3 (CRISP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Secretory Protein 3 (CRISP3) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Secretory Protein 3 (CRISP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes CRISP3 Kits ELISA
    CRISP3 (Cysteine-Rich Secretory Protein 3 (CRISP3))
    Autre désignation
    Cysteine Rich Secretory Protein 3 (CRISP3 Produits)
    Synonymes
    aln-a Kit ELISA, crisp3 Kit ELISA, MGC76177 Kit ELISA, CRISP3 Kit ELISA, Aeg2 Kit ELISA, CRISP-3 Kit ELISA, CRS3 Kit ELISA, SGP28 Kit ELISA, dJ442L6.3 Kit ELISA, AEG2 Kit ELISA, HSP-3 Kit ELISA, cysteine-rich secretory protein 3 Kit ELISA, cysteine-rich secretory protein 3 S homeolog Kit ELISA, cysteine rich secretory protein 3 Kit ELISA, cysteine-rich secretory protein 2 Kit ELISA, CRISP3 Kit ELISA, crisp3.S Kit ELISA, crisp3 Kit ELISA, CpipJ_CPIJ001507 Kit ELISA, Crisp3 Kit ELISA, LOC481834 Kit ELISA
    Sujet

    Gene Name: Cysteine Rich Secretory Protein 3

    Gene Aliases: CRS3, Aeg2, CRISP-3, SGP28, Specific granule protein of 28 kDa

    ID gène
    10321
    UniProt
    P54108
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