CDA Kit ELISA

N° du produit ABIN5653477

Détail du produit CDA Kit ELISA

Antigène: CDA (Cytidine Deaminase (CDA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cytidine Deaminase (CDA). No significant cross-reactivity or interference between Cytidine Deaminase (CDA) and analogues was observed.

Sensibilité: 0.32 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytidine Deaminase (CDA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytidine Deaminase (CDA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytidine Deaminase (CDA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytidine Deaminase (CDA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytidine Deaminase (CDA) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytidine Deaminase (CDA) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CDA (Cytidine Deaminase (CDA))

Autre désignation: Cytidine Deaminase (CDA Produits)

Synonymes: CDA Kit ELISA, cda Kit ELISA, zgc:77948 Kit ELISA, DDBDRAFT_0188181 Kit ELISA, DDBDRAFT_0230205 Kit ELISA, DDB_0188181 Kit ELISA, DDB_0230205 Kit ELISA, CDD Kit ELISA, 2210401N16Rik Kit ELISA, cytidine deaminase Kit ELISA, cytidine aminohydrolase; CDA Kit ELISA, cytidine deaminase b Kit ELISA, Cytidine deaminase Kit ELISA, cytidine deaminase Cdd Kit ELISA, CDA Kit ELISA, cda Kit ELISA, VNG_RS03500 Kit ELISA, OE_RS03485 Kit ELISA, CDD1 Kit ELISA, cdd Kit ELISA, cdab Kit ELISA, cdd-1 Kit ELISA, cdd-2 Kit ELISA, CNC04940 Kit ELISA, Tb09.160.1680 Kit ELISA, Cda Kit ELISA

Sujet:

Gene Name: Cytidine Deaminase

Gene Aliases: CDD, Cytidine aminohydrolase

ID gène: 978

UniProt: P32320