CYBB Kit ELISA

N° du produit ABIN5653479

Détail du produit CYBB Kit ELISA

Antigène: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cytochrome b-245 Beta Polypeptide (CYBb). No significant cross-reactivity or interference between Cytochrome b-245 Beta Polypeptide (CYBb) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome b-245 Beta Polypeptide (CYBb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome b-245 Beta Polypeptide (CYBb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome b-245 Beta Polypeptide (CYBb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome b-245 Beta Polypeptide (CYBb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome b-245 Beta Polypeptide (CYBb) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome b-245 Beta Polypeptide (CYBb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CYBB (Cytochrome B-245, beta Polypeptide (CYBB))

Autre désignation: Cytochrome b-245 Beta Polypeptide (CYBB Produits)

Synonymes: zgc:64144 Kit ELISA, cgd Kit ELISA, gp91-1 Kit ELISA, gp91-phox Kit ELISA, gp91phox Kit ELISA, nox2 Kit ELISA, NOX2 Kit ELISA, cybb Kit ELISA, NOX1 Kit ELISA, AMCBX2 Kit ELISA, CGD Kit ELISA, GP91-1 Kit ELISA, GP91-PHOX Kit ELISA, GP91PHOX Kit ELISA, p91-PHOX Kit ELISA, Nox2 Kit ELISA, C88302 Kit ELISA, CGD91-phox Kit ELISA, Cgd Kit ELISA, Cyd Kit ELISA, Gp91-phox Kit ELISA, CYBB Kit ELISA, cytochrome b-245, beta polypeptide (chronic granulomatous disease) Kit ELISA, cytochrome b-245, beta polypeptide Kit ELISA, cytochrome b-245 heavy chain Kit ELISA, cytochrome b-245, beta polypeptide L homeolog Kit ELISA, cytochrome b-245 beta polypeptide Kit ELISA, cytochrome b-245 beta chain Kit ELISA, NADPH oxidase heavy chain subunit Kit ELISA, cybb Kit ELISA, CYBB Kit ELISA, cybb.L Kit ELISA, Cybb Kit ELISA, GP91-PHOX Kit ELISA

Sujet:

Gene Name: Cytochrome b-245 Beta Polypeptide

Gene Aliases: CGD, GP91PHOX, NOX2, Neutrophil cytochrome b91, Cytochrome b558 beta, Heme-binding membrane glycoprotein gp91phox, Superoxide-generating NADPH oxidase heavy chain

ID gène: 1536

UniProt: P04839