COX IV Kit ELISA

N° du produit ABIN5653486

Détail du produit COX IV Kit ELISA

Antigène: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). No significant cross-reactivity or interference between Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Autre désignation: Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1 Produits)

Synonymes: COX4 Kit ELISA, COX4-1 Kit ELISA, COXIV Kit ELISA, mg:bb02d03 Kit ELISA, zgc:110058 Kit ELISA, GB20012 Kit ELISA, AL024441 Kit ELISA, Cox4 Kit ELISA, Cox4a Kit ELISA, cox4 Kit ELISA, cox4i1 Kit ELISA, coxiv Kit ELISA, BcDNA:AT07685 Kit ELISA, CG10396 Kit ELISA, COX IV Kit ELISA, CX41 Kit ELISA, CX42 Kit ELISA, Dmel\CG10396 Kit ELISA, IV Kit ELISA, cytochrome c oxidase subunit 4I1 Kit ELISA, cytochrome c oxidase subunit IV isoform 1 Kit ELISA, cytochrome c oxidase subunit 4 isoform 1, mitochondrial Kit ELISA, cytochrome c oxidase polypeptide IV Kit ELISA, cytochrome c oxidase subunit IV Kit ELISA, cytochrome c oxidase subunit 4i1 Kit ELISA, cytochrome c oxidase subunit IV isoform 1 S homeolog Kit ELISA, Cytochrome c oxidase subunit 4-like Kit ELISA, COX4I1 Kit ELISA, cox4i1 Kit ELISA, LOC412396 Kit ELISA, Cox4I1 Kit ELISA, LOC780848 Kit ELISA, BMEII0322 Kit ELISA, RSal33209_1582 Kit ELISA, SJAG_00976 Kit ELISA, HMPREF0733_11926 Kit ELISA, Cox4i1 Kit ELISA, cox4i1.S Kit ELISA, COX4L Kit ELISA

Sujet:

Gene Name: Cytochrome C Oxidase Subunit IV Isoform 1

Gene Aliases: COX4, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial

Pathways: Proton Transport