CYP3A4 Kit ELISA
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- Antigène Voir toutes CYP3A4 Kits ELISA
- CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.312 ng/mL - 20 ng/mL
- Seuil minimal de détection
- 0.312 ng/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 3A4 (CYP3A4). No significant cross-reactivity or interference between Cytochrome P450 3A4 (CYP3A4) and analogues was observed.
- Sensibilité
- 0.122 ng/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 3A4 (CYP3A4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 3A4 (CYP3A4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 3A4 (CYP3A4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 3A4 (CYP3A4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 3A4 (CYP3A4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 3A4 (CYP3A4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes CYP3A4 Kits ELISA
- CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))
- Autre désignation
- Cytochrome P450 3A4 (CYP3A4 Produits)
- Synonymes
- CP33 Kit ELISA, CP34 Kit ELISA, CYP3A Kit ELISA, CYP3A3 Kit ELISA, CYPIIIA3 Kit ELISA, CYPIIIA4 Kit ELISA, HLP Kit ELISA, NF-25 Kit ELISA, P450C3 Kit ELISA, P450PCN1 Kit ELISA, MGC108372 Kit ELISA, CYP3A80 Kit ELISA, CYP3A4 Kit ELISA, CYP3A21 Kit ELISA, CYPIIIA21 Kit ELISA, CYP3A12 Kit ELISA, cytochrome P450 family 3 subfamily A member 4 Kit ELISA, cytochrome P450 family 3 subfamily A member 43 Kit ELISA, cytochrome P450 3A4 Kit ELISA, cytochrome P450, subfamily IIIA, polypeptide 4 Kit ELISA, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 Kit ELISA, cytochrome P450, family 3, subfamily A, polypeptide 4 Kit ELISA, CYP3A4 Kit ELISA, CYP3A43 Kit ELISA, PTRG_01782 Kit ELISA, PTRG_06060 Kit ELISA, cyp3a4 Kit ELISA
- Sujet
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Gene Name: Cytochrome P450 3A4
Gene Aliases: CYP3A3, CYPIIIA4, 1,8-cineole 2-exo-monooxygenase, Albendazole sulfoxidase, Nifedipine oxidase, Quinine 3-monooxygenase, Taurochenodeoxycholate 6-alpha-hydroxylase
- ID gène
- 1576
- UniProt
- P08684
- Pathways
- Steroid Hormone Biosynthesis
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