DAP Kinase 1 Kit ELISA

N° du produit ABIN5653555

Détail du produit DAP Kinase 1 Kit ELISA

Antigène: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Death Associated Protein Kinase 1 (DAPK1). No significant cross-reactivity or interference between Death Associated Protein Kinase 1 (DAPK1) and analogues was observed.

Sensibilité: 0.062 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Death Associated Protein Kinase 1 (DAPK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Death Associated Protein Kinase 1 (DAPK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Death Associated Protein Kinase 1 (DAPK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Death Associated Protein Kinase 1 (DAPK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Death Associated Protein Kinase 1 (DAPK1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Death Associated Protein Kinase 1 (DAPK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DAP Kinase 1 (DAPK1) (Death-Associated Protein Kinase 1 (DAPK1))

Autre désignation: Death Associated Protein Kinase 1 (DAPK1 Produits)

Synonymes: DAPK1 Kit ELISA, MGC81366 Kit ELISA, si:ch211-66i11.1 Kit ELISA, wu:fj09c03 Kit ELISA, dapk Kit ELISA, MGC83745 Kit ELISA, 2310039H24Rik Kit ELISA, 2810425C21Rik Kit ELISA, AI642003 Kit ELISA, D13Ucla1 Kit ELISA, DAP-Kinase Kit ELISA, DAPK Kit ELISA, death-associated protein kinase 1 Kit ELISA, death associated protein kinase 1 L homeolog Kit ELISA, death associated protein kinase 1 Kit ELISA, death associated protein kinase 1 S homeolog Kit ELISA, LOC427465 Kit ELISA, dapk1.L Kit ELISA, DAPK1 Kit ELISA, dapk1 Kit ELISA, dapk1.S Kit ELISA, Dapk1 Kit ELISA

Sujet:

Gene Name: Death Associated Protein Kinase 1

Pathways: Signalisation MAPK, Interferon-gamma Pathway