DIDO1 Kit ELISA

N° du produit ABIN5653558

Détail du produit DIDO1 Kit ELISA

Antigène: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Death Inducer Obliterator 1 (DIDO1). No significant cross-reactivity or interference between Death Inducer Obliterator 1 (DIDO1) and analogues was observed.

Sensibilité: 0.104 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Death Inducer Obliterator 1 (DIDO1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Death Inducer Obliterator 1 (DIDO1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Death Inducer Obliterator 1 (DIDO1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Death Inducer Obliterator 1 (DIDO1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Death Inducer Obliterator 1 (DIDO1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Death Inducer Obliterator 1 (DIDO1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DIDO1 (Death Inducer-Obliterator 1 (DIDO1))

Autre désignation: Death Inducer Obliterator 1 (DIDO1 Produits)

Synonymes: 5DI Kit ELISA, TXDI1 Kit ELISA, BYE1 Kit ELISA, C20orf158 Kit ELISA, DATF-1 Kit ELISA, DATF1 Kit ELISA, DIDO2 Kit ELISA, DIDO3 Kit ELISA, DIO-1 Kit ELISA, DIO1 Kit ELISA, dJ885L7.8 Kit ELISA, 6720461J16Rik Kit ELISA, C530043I07 Kit ELISA, D130048F08Rik Kit ELISA, Datf1 Kit ELISA, Dido2 Kit ELISA, Dido3 Kit ELISA, mKIAA0333 Kit ELISA, DIDO1 Kit ELISA, datf1l Kit ELISA, fi35b04 Kit ELISA, wu:fi35b04 Kit ELISA, zgc:158157 Kit ELISA, DKFZp469J1523 Kit ELISA, bye1 Kit ELISA, dio1 Kit ELISA, datf1 Kit ELISA, dido2 Kit ELISA, dido3 Kit ELISA, dio-1 Kit ELISA, iodothyronine deiodinase 1 Kit ELISA, death inducer-obliterator 1 Kit ELISA, death inducer-obliterator 1 L homeolog Kit ELISA, DIO1 Kit ELISA, DIDO1 Kit ELISA, Dido1 Kit ELISA, dido1.L Kit ELISA, dido1 Kit ELISA

Sujet:

Gene Name: Death Inducer Obliterator 1

Gene Aliases: BYE1, DATF1, DIDO2, DIDO3, DIO1, Death Associated Transcription Factor 1

ID gène: 11083

UniProt: Q9BTC0

Pathways: Hormone Activity